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Recombinant Interleukin 4 (IL-4), human

Catalogue Number CYT-211
Synonyms BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Introductionn IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Description Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton.
The IL-4 is purified by proprietary chromatographic techniques.
Source Escherichia Coli. Also available from HEK293 and CHO cells
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Solubility It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp.
Biological Activity The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.
Protein content Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.

1. Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation1
Publication:The Journal of Immunology, 2006, 177: 6695-6704.Copyright © 2006 by The American Association of Immunologists, Inc.

2.Title:CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse
Publication:Published online before print June 27, 2005, doi: 10.1073/pnas.0501050102 PNAS July 5, 2005 vol. 102 no. 27 9583-9588

3.Title:A Network Modeling Approach to Analysis of the Th2 Memory Responses Underlying Human Atopic Disease
Publication:doi: 10.4049/?jimmunol.0804125 The Journal of Immunology May 15, 2009 vol. 182 no. 10 6011-6021

4.Title:Defective IL-10 production in severe phenotypes of Crohn’s disease
Publication:Published online before print February 23, 2009, doi: 10.1189/jlb.1108698 May 2009 Journal of Leukocyte Biology vol. 85 no. 5 896-903

5.Title:Adenosine deaminase potentiates the generation of effector, memory, and regulatory CD4+ T cells .
Publication:Published online before print October 19, 2010, doi: 10.1189/jlb.1009696 January 2011 Journal of Leukocyte Biology vol. 89 no. 1 127-136 .

6.Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation/

7.Title:Airway Epithelial Cells Regulate the Functional Phenotype of Locally Differentiating Dendritic Cells: Implications for the Pathogenesis of Infectious and Allergic Airway Disease.
Publication: The Journal of Immunology January 1, 2009 vol. 182 no. 1 72-83

Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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