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Recombinant adiponectin (acrp30), human, from HEK 293 cells
| Catalogue Number | CYT-434 |
| Synonyms | Acrp30, AdipoQ, GBP-28, APM-1, ACDC. |
| Introduction | Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. |
| Patent Rights | The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A). |
| Description | The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag. |
| Source | HEK293 (Human Embryonic Kidney cell line). Also available from E. coli and in trimeric form. |
| Activity in vitro | In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED50 was ~6 µg/ml. |
| Formulation | Lyophilized from 0.5 mg/ml in PBS buffer. |
| Solubility | Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. |
| Stability | For long term, store lyophilized AdipoQ at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable for 24 months when stored at -20°C. |
| Purity | Greater than 98% as determined by HPLC and SDS PAGE. |
| Applications | ELISA, Western blotting, cell culture and/or animal studies. |
| Amino Acid Sequence | ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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