| Kisker | Berthold DS | Titertek | USBio | Biontex | ProSpec |
| Genecraft | Biothema | EuroBioSciences | Biosera | Genelink | ID Labs |
METAFECTENE® SI+
Para transfección de miRNA y siRNA el reactivo Metafectene SI+ es la solución ideal.
Highlights
- Now protocol optimized for "fast-forward"
- Specific optimization of lipid composition for RNA transfections (siCOM Technology)
- Integrated RMA and TOP Technology
- Efficient knockdown and low siRNA volume
- Rapid protocol: two interdependent assays within one week*
- Protocol extremely suitable for screening processes
METAFECTENE® SI+ is a lipid compound designed and optimized especially for siRNA and miRNA transfection (siCOM Technology). The characteristic second-generation features of Biontex's patented and proven RMA and TOP technologies deliver high knockdown rates, low RNA concentration and very low toxicity, enabling them to be used for all cell lines and primary cells.
Main advantages of the new "fast-forward" optimized protocol:
- Substantial reduction of required lipid amounts
- Up to 4-point increase of lipid mileage
- Even more reduction of toxicity effects
Compatibility:
If you already use a satisfactory optimized protocol with METAFECTENE® SI, this is fully compatible with METAFECTENE® SI+. However, we would recommend that you perform reevaluation to ensure the best possible results, given the low effort involved in the procedure.
This new development also permits a reverse protocol which is simplified and one day shorter than conventional protocols. In addition, the product uses a predefined ratio of RNA to lipids, rendering complex optimization of the lipoplex compound unnecessary. Because of the time saving from four days to three, two successive result-dependent transfections can be performed within a single working week*.
Screening processes benefit both from this timesaving and from the fact that the lipoplex formation, and thus all pipetting steps, take place in the well of a multiwell culture plate, eliminating the need for rinsing or transfer. siRNA or miRNA transfection can thus be performed during screening with the maximum efficiency and ease.
| Product Specifications | |
|---|---|
| Application | Transfection of eukariotic cells with siRNA or miRNA |
| Formulation | Cationic lipids with colipids in water |
| Content |
Lipid formulation / sufficient quantity of SI+ buffer |
| Assays | Up to 4000 (96-well) or 650 (24-well) per 1 ml reagent |
| Shipping | At room temperature |
| Storage | ≤ –15°C |
| Shelf Life | 1 year (after date of delivery) |
* At max. 48-hour incubation period between lipoplex addition and evaluation.